When I came up with this theme, I felt that I should make it a bit more of a challenge to draft because there are so many characters that are little sisters out there. Little sisters with an older brother, a twin sister, or multiple siblings are quite prevalent in anime. The direction I went with the little sister only having a non-twin older sister for a sibling is not that commonplace in anime. There are plenty of good choices to go with, but not so many that the theme becomes too broad.

One other thing that I am trying to do within this theme is not to have anyone double-cast on either side (if the series also has an English Dub). So my cast so far goes like this:

Houki Shinonono (Infinite Stratos) - Yōko Hikasa / Monica Rial
Lina Inverse (Slayers) - Megumi Hayashibara / Lisa Ortiz (TV), Cynthia Martinez (OVA and Movies)
Kaoru Akashi (Zettai Karen Children / Psychic Squad) - Aya Hirano
Fuuko Ibuki (Clannad) - Ai Nonaka / Hilary Haag
Yune (Croisée in a Foreign Labyrinth) - Nao Tōyama
Yutaka Kobayakawa (Lucky Star) - Shizuka Hasegawa / Hynden Walch
Haruna Sairenji (To Love-Ru) - Sayuri Yahagi
Shiori Misaka (Kanon) - Akemi Satou / Maggie Flecknoe
Miharu Sena Kanaka (Girls Bravo) - Ayako Kawasumi / Michelle Ruff
Kano Kirishima (Air) - Asami Okamoto / Stephanie Wittels
Chika (Hidamari Sketch) - Rie Kugimiya

The final pick went to the opposite side of the coin since the little sister of this pair was already taken at the start of the draft, so with her older sister still sitting out there three picks from the end of the draft I take for my final character and name the team Little Sisters to the Rescue:

Yui Hirasawa (K-On!) - Aki Toyosaki / Stephanie Sheh

And now that the drafting is over, I can say who the older sisters are for the first ten little sisters. Both of the last two sisters (Chika and Yui Hirasawa) had their opposite sisters drafted by Serial Experiments Nobue (Sae and Ui Hirasawa).

Houki Shinonono ---> Tabane Shinonono
Lina Inverse ---> Luna Inverse
Kaoru Akashi ---> Yoshimi Akashi
Fuuko Ibuki ---> Kouko Ibuki-Yoshino
Yune ---> Shione
Yutaka Kobayakawa ---> Yui Kobayakawa-Narumi
Haruna Sairenji ---> Akiho Sairenji
Shiori Misaka ---> Kaori Misaka
Miharu Sena Kanaka ---> Maharu Sena Kanaka
Kano Kirishima ---> Hijiri Kirishima